Nonalcoholic fatty liver, as the manifestation of metabolic syndrome in liver, is closely related to obesity, insulin resistance (IR), diabetes, hyperlipidemia and other metabolic abnormalities. NAFLD is a clinicopathologic syndrome characterized by hepatic parenchymal cell steatosis and fat storage. The disease spectrum includes simple hepatic steatohepatitis, non-alcoholic steatohepatitis (NASH), liver fibrosis, which can progress to cirrhosis and liver cancer.

Molding methods: High fat diet was given and CCl4 was injected subcutaneously to induce liver fibrosis and fatty degeneration.

Detection index:

Weekly measurement: weight, food intake;

Regular tests:liver function, liver B-ultrasound, liver puncture pathology.

1. The HE staining of liver tissue showed fatty degeneration at 3 months after modeling.

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     Figure 1. The HE staining of liver tissue in cynomolgus monkey with non-acoholic Fatty Liver.

2. The HE staining showed fat vacuolization and extensive infiltration of inflammatory cells at 5-6 months after modeling.

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     Figure 2. The HE staining of liver tissue in cynomolgus monkey with non-alcoholic Steatohepatitis.

3. The VG staining of liver tissue showed destruction of lobular structure and formation of fibrous septa at 8-9 months after modeling.

    Figure 3. The VG staining of liver tissue in cynomolgus monkey with non-alcoholic liver fibrosis.